| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
ORIGINAL RESEARCH |
From the Divisions of Maternal-Fetal Medicine and Reproductive Endocrinology, Department of Obstetrics and Gynecology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
Address reprint requests to: Alexander D. Allaire, MD, MSPH Department of Obstetrics and Gynecology University of North Carolina, Chapel Hill 214 MacNider Campus Box #7570 Chapel Hill, NC 27599-7570 E-mail: allaire{at}med.unc.edu
Objective: To determine whether preeclampsia is associated with an increase in placental apoptosis and differential expression of mediators of apoptosis.
Methods: Placental samples from 31 preeclamptic women and 31 normotensive controls were analyzed using terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick-end labeling staining. Expression of Fas, Fas ligand, Bcl-2, and Bax was assessed using immunohistochemistry.
Results: The median percent apoptotic nuclei was significantly higher for the study group than for the controls (0.49 versus 0.19; P = .001), as was the median percent apoptotic nuclei in the trophoblast nuclei (0.33 versus 0.09; P < .01). Fas ligand expression was significantly less and Fas expression significantly greater in the villus trophoblast among the study subjects compared with controls. There was no difference in the expression of Bax or Bcl-2 between groups.
Conclusion: Placental apoptosis and altered expression of Fas and Fas ligand in trophoblast might influence pathogenesis or sequelae of preeclampsia.
This article has been cited by other articles:
|
|
 |
|
  J. Detmar, M. Y. Rennie, K. J. Whiteley, D. Qu, Y. Taniuchi, X. Shang, R. F. Casper, S. L. Adamson, J. G. Sled, and A. Jurisicova Fetal growth restriction triggered by polycyclic aromatic hydrocarbons is associated with altered placental vasculature and AhR-dependent changes in cell death Am J Physiol Endocrinol Metab, August 1, 2008; 295(2): E519 - E530. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Forbes, M. Westwood, P. N. Baker, and J. D. Aplin Insulin-like growth factor I and II regulate the life cycle of trophoblast in the developing human placenta Am J Physiol Cell Physiol, June 1, 2008; 294(6): C1313 - C1322. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Humphrey, C. Sonnenberg-Hirche, S. D. Smith, C. Hu, A. Barton, Y. Sadovsky, and D. M. Nelson Epidermal Growth Factor Abrogates Hypoxia-Induced Apoptosis in Cultured Human Trophoblasts through Phosphorylation of BAD Serine 112 Endocrinology, May 1, 2008; 149(5): 2131 - 2137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. M. Abrahams, P. B. Aldo, S. P. Murphy, I. Visintin, K. Koga, G. Wilson, R. Romero, S. Sharma, and G. Mor TLR6 Modulates First Trimester Trophoblast Responses to Peptidoglycan J. Immunol., May 1, 2008; 180(9): 6035 - 6043. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Cindrova-Davies, H.-W. Yung, J. Johns, O. Spasic-Boskovic, S. Korolchuk, E. Jauniaux, G. J. Burton, and D. S. Charnock-Jones Oxidative Stress, Gene Expression, and Protein Changes Induced in the Human Placenta during Labor Am. J. Pathol., October 1, 2007; 171(4): 1168 - 1179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Soleymanlou, A. Jurisicova, Y. Wu, M. Chijiiwa, J. E. Ray, J. Detmar, T. Todros, S. Zamudio, M. Post, and I. Caniggia Hypoxic Switch in Mitochondrial Myeloid Cell Leukemia Factor-1/Mtd Apoptotic Rheostat Contributes to Human Trophoblast Cell Death in Preeclampsia Am. J. Pathol., August 1, 2007; 171(2): 496 - 506. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Cervar-Zivkovic, C. Hu, A. Barton, Y. Sadovsky, G. Desoye, U. Lang, and D.M. Nelson Endothelin-1 Attenuates Apoptosis in Cultured Trophoblasts From Term Human Placentas Reproductive Sciences, July 1, 2007; 14(5): 430 - 439. [Abstract] [PDF]
|
|
|
|
 |
|
 S. L. Straszewski-Chavez, I. P. Visintin, N. Karassina, G. Los, P. Liston, R. Halaban, A. Fadiel, and G. Mor XAF1 Mediates Tumor Necrosis Factor-{alpha}-induced Apoptosis and X-linked Inhibitor of Apoptosis Cleavage by Acting through the Mitochondrial Pathway J. Biol. Chem., April 27, 2007; 282(17): 13059 - 13072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Belkacemi, S. A. Bainbridge, M. A. Dickinson, G. N. Smith, and C. H. Graham Glyceryl Trinitrate Inhibits Hypoxia/Reoxygenation-Induced Apoptosis in the Syncytiotrophoblast of the Human Placenta: Therapeutic Implications for Preeclampsia Am. J. Pathol., March 1, 2007; 170(3): 909 - 920. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Wang, K. W. Yim, T. C.W. Poon, K. W. Choy, C. Y. Chu, W. T. Lui, T. K. Lau, M. S. Rogers, and T. N. Leung Innate Immune Response by Ficolin Binding in Apoptotic Placenta Is Associated with the Clinical Syndrome of Preeclampsia Clin. Chem., January 1, 2007; 53(1): 42 - 52. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Bechi, F. Ietta, R. Romagnoli, S. Focardi, I. Corsi, C. Buffi, and L. Paulesu Estrogen-Like Response to p-Nonylphenol in Human First Trimester Placenta and BeWo Choriocarcinoma Cells Toxicol. Sci., September 1, 2006; 93(1): 75 - 81. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Bainbridge, L. Belkacemi, M. Dickinson, C. H. Graham, and G. N. Smith Carbon Monoxide Inhibits Hypoxia/Reoxygenation-Induced Apoptosis and Secondary Necrosis in Syncytiotrophoblast Am. J. Pathol., September 1, 2006; 169(3): 774 - 783. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Tjoa, T. Cindrova-Davies, O. Spasic-Boskovic, D. W. Bianchi, and G. J. Burton Trophoblastic Oxidative Stress and the Release of Cell-Free Feto-Placental DNA Am. J. Pathol., August 1, 2006; 169(2): 400 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Equils, D. Lu, M. Gatter, S. S. Witkin, C. Bertolotto, M. Arditi, J. A. McGregor, C. F. Simmons, and C. J. Hobel Chlamydia Heat Shock Protein 60 Induces Trophoblast Apoptosis through TLR4 J. Immunol., July 15, 2006; 177(2): 1257 - 1263. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Rovere-Querini, S. Antonacci, G. Dell'Antonio, A. Angeli, G. Almirante, E. D. Cin, L. Valsecchi, C. Lanzani, M. G. Sabbadini, C. Doglioni, et al. Plasma and tissue expression of the long pentraxin 3 during normal pregnancy and preeclampsia. Obstet. Gynecol., July 1, 2006; 108(1): 148 - 155. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. A. Hills, V. M. Abrahams, B. Gonzalez-Timon, J. Francis, B. Cloke, L. Hinkson, R. Rai, G. Mor, L. Regan, M. Sullivan, et al. Heparin prevents programmed cell death in human trophoblast Mol. Hum. Reprod., April 1, 2006; 12(4): 237 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Sziller, P. Hupuczi, N. Normand, A. Halmos, Z. Papp, and S. S. Witkin Fas (TNFRSF6) Gene Polymorphism in Pregnant Women With Hemolysis, Elevated Liver Enzymes, and Low Platelets and in Their Neonates. Obstet. Gynecol., March 1, 2006; 107(3): 582 - 587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Vasarhelyi, A. Cseh, I. Kocsis, A. Treszl, B. Gyorffy, and J. Rigo Jr Three mechanisms in the pathogenesis of pre-eclampsia suggested by over-represented transcription factor-binding sites detected with comparative promoter analysis Mol. Hum. Reprod., January 1, 2006; 12(1): 31 - 34. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Straszewski-Chavez, V. M. Abrahams, and G. Mor The Role of Apoptosis in the Regulation of Trophoblast Survival and Differentiation during Pregnancy Endocr. Rev., December 1, 2005; 26(7): 877 - 897. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Roberts and H. S. Gammill Preeclampsia: Recent Insights Hypertension, December 1, 2005; 46(6): 1243 - 1249. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Li, J. Dakour, L. J. Guilbert, B. Winkler-Lowen, F. Lyall, and D. W. Morrish PL74, a Novel Member of the Transforming Growth Factor-{beta} Superfamily, Is Overexpressed in Preeclampsia and Causes Apoptosis in Trophoblast Cells J. Clin. Endocrinol. Metab., May 1, 2005; 90(5): 3045 - 3053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Sziller, D. Nguyen, A. Halmos, P. Hupuczi, Z. Papp, and S. S. Witkin An A>G polymorphism at position -670 in the Fas (TNFRSF6) gene in pregnant women with pre-eclampsia and intrauterine growth restriction Mol. Hum. Reprod., March 1, 2005; 11(3): 207 - 210. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Daayana, P. Bakerer, and I. Crocker An Image Analysis Technique for the Investigation of Variations in Placental Morphology in Pregnancies Complicated by Preeclampsia With and Without Interauterine Growth Restriction Reproductive Sciences, December 1, 2004; 11(8): 545 - 552. [Abstract] [PDF]
|
|
|
|
|
|
V. M. Abrahams, P. Bole-Aldo, Y. M. Kim, S. L. Straszewski-Chavez, T. Chaiworapongsa, R. Romero, and G. Mor Divergent Trophoblast Responses to Bacterial Products Mediated by TLRs J. Immunol., October 1, 2004; 173(7): 4286 - 4296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Huppertz and J. C. P. Kingdom Apoptosis in the Trophoblast--Role of Apoptosis in Placental Morphogenesis Reproductive Sciences, September 1, 2004; 11(6): 353 - 362. [Abstract] [PDF]
|
|
|
|
|
|
S. L. Straszewski-Chavez, V. M. Abrahams, E. F. Funai, and G. Mor X-linked inhibitor of apoptosis (XIAP) confers human trophoblast cell resistance to Fas-mediated apoptosis Mol. Hum. Reprod., January 1, 2004; 10(1): 33 - 41. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. R Balkundi, J. A Ziegler, J. F Watchko, C. Craven, and M. Trucco Regulation of FasL/Fas in Human Trophoblasts: Possible Implications for Chorioamnionitis Biol Reprod, August 1, 2003; 69(2): 718 - 724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Mu, T. Kanzaki, X. Si, T. Tomimatsu, H. Fukuda, M. Shioji, Y. Murata, Y. Sugimoto, and A. Ichikawa Apoptosis and Related Proteins in Placenta of Intrauterine Fetal Death in Prostaglandin F Receptor-Deficient Mice Biol Reprod, June 1, 2003; 68(6): 1968 - 1974. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. P. Crocker, S. Cooper, S. C. Ong, and P. N. Baker Differences in Apoptotic Susceptibility of Cytotrophoblasts and Syncytiotrophoblasts in Normal Pregnancy to Those Complicated with Preeclampsia and Intrauterine Growth Restriction Am. J. Pathol., February 1, 2003; 162(2): 637 - 643. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.-H. Hung, J. N. Skepper, D. S. Charnock-Jones, and G. J. Burton Hypoxia-Reoxygenation: A Potent Inducer of Apoptotic Changes in the Human Placenta and Possible Etiological Factor in Preeclampsia Circ. Res., June 28, 2002; 90(12): 1274 - 1281. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Aschkenazi, S. Straszewski, K. M.A. Verwer, H. Foellmer, T. Rutherford, and G. Mor Differential Regulation and Function of the Fas/Fas Ligand System in Human Trophoblast Cells Biol Reprod, June 1, 2002; 66(6): 1853 - 1861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Black, H. Yu, J. Lee, M. Sachchithananthan, and R. L. Medcalf Physiologic Concentrations of Magnesium and Placental Apoptosis: Prevention by Antioxidants Obstet. Gynecol., August 1, 2001; 98(2): 319 - 324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-D. HSU, H. HARIRAH, H. BASHERRA, and G. MOR Serum Soluble Fas Levels in Preeclampsia Obstet. Gynecol., April 1, 2001; 97(4): 530 - 532. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
