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Rapid hCG-Specific Radioimmunoassay for Menstrual Aspiration

From the Department of Surgery (Gyn), Peter Bent Brigham Hospital and the Harvard Medical School, Boston, Massachusetts, and the Department of Obstetrics and Gynecology, Kapiolani Maternity Hospital and the University of Hawaii Medical School, Hawaii.

Fifty-one women with missed periods ranging from 5 to 14 days who suspected a pregnancy underwent an aspiration currettage with a flexible soft-tipped cannula attached to a Karman-type syringe. Sera from these women were measured for human chorionic gonadotropin (hCG) by a rapid 2-hour solid phase radioimmunoassay using an antibody with a specificity for the beta subunit of hCG. The sensitivity and specificity of this assay insured detection of hCG with 100% accuracy at the time of the missed period. Of the 51 women, 33 (61.7%) were found to have hCG in their serum, and 29 (56.8%) had histologic evidence diagnostic of pregnancy.